Detailed information of TA system TA158762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 329..803
• Replicon: plasmid pHNBYF36-3
• Accession: NZ_CP054132
• Organism: Citrobacter portucalensis strain BY9F36

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: HRV88_RS24175
• Protein ID: WP_000262326.1
• Coordinates: 537..803 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: HRV88_RS24170
• Protein ID: WP_000879771.1
• Coordinates: 329..553 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRV88_RS24170 (HRV88_24100)	329..553	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
HRV88_RS24175 (HRV88_24105)	537..803	+	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRV88_RS24180 (HRV88_24110)	865..1344	-	480	WP_227525340.1	hypothetical protein	-
HRV88_RS24185 (HRV88_24115)	1436..1867	-	432	WP_053274236.1	DUF6530 family protein	-
HRV88_RS24190 (HRV88_24120)	3039..3239	+	201	WP_024148903.1	hypothetical protein	-
HRV88_RS24195 (HRV88_24125)	3287..3475	-	189	WP_000165998.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..3692	3692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T158762 WP_000262326.1 NZ_CP054132:537-803 [Citrobacter portucalensis]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T158762 NZ_CP069810:1744032-1744451 [Cupriavidus oxalaticus]
ATGATTGTCTTGGATACGAATGTCGTCTCCGAGGCGATGAAGCCCGAACCTAGCCCGGCCGTGCAAGCTTGGTTGAATGA
GCAGGTAGCGGAAACCCTCTACCTGTCCAGCGTGACGCTGGCCGAACTGCTGTTCGGCATCGGCGCGCTGCCGAACGGGA
AACGTAAGAAAGGCCTGGGCGAAGCCCTGGACGGCTTGCTCGAACTGTTCGGCGAGCGGGTGCTGATGTTCGACACCGAA
GCAGCTCGCCATTATGCCGAGCTGGCGGCGAAGGCCCGCACAGCCGGGAAGGGTTTTCCAACACCCCACGGATATATTGG
GGCTATTGCGGCTTCCAAGGGATTTATCGTAGCAACACGCGACACCAGCCCTTTCGAAGCCGCTGGGCTTACTGTTATCA
ATCCTTGGAACCATCAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT158762 WP_000879771.1 NZ_CP054132:329-553 [Citrobacter portucalensis]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT158762 NZ_CP069810:1743781-1744035 [Cupriavidus oxalaticus]
ATGGCAATCCTGACAGTGCGGAATGTGCCCGATGAGGTGCATCGCGCGCTGCGCTTACGGGCCGCCGAGCATGGCCGCAG
TACAGAGGCGGAGGTGCGCGCGATTCTGGAAAGTGCAGTGAAGTCGGAAAAGCGCATCCGCATGGGCGACGCTCTGGCGG
AGCTTGGCCGTCGCGTAGGGCTGACAAACGATGATTTTGCAGTGTTGGACCAGGTGCGCGACAAGGTGCCGGCAGAACCC
ATGAGGTTTGAATGA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5