Detailed information of TA system TA158757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4256690..4257266
• Replicon: chromosome
• Accession: NZ_CP054129
• Organism: Citrobacter portucalensis strain BY9F36

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HRV88_RS20310
• Protein ID: WP_128612218.1
• Coordinates: 4256979..4257266 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HRV88_RS20305
• Protein ID: WP_128612219.1
• Coordinates: 4256690..4256992 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRV88_RS20285 (HRV88_20240)	4252378..4252920	+	543	WP_003830032.1	hypothetical protein	-
HRV88_RS20290 (HRV88_20245)	4253210..4255360	+	2151	WP_003830033.1	pyruvate/proton symporter BtsT	-
HRV88_RS20295 (HRV88_20250)	4255411..4255605	+	195	WP_003830038.1	YbdD/YjiX family protein	-
HRV88_RS20300 (HRV88_20255)	4255618..4256574	+	957	WP_235125540.1	GTPase	-
HRV88_RS20305 (HRV88_20260)	4256690..4256992	-	303	WP_128612219.1	BrnA antitoxin family protein	Antitoxin
HRV88_RS20310 (HRV88_20265)	4256979..4257266	-	288	WP_128612218.1	BrnT family toxin	Toxin
HRV88_RS20315 (HRV88_20270)	4257620..4259965	+	2346	WP_235125542.1	class I SAM-dependent DNA methyltransferase	-
HRV88_RS20320 (HRV88_20275)	4259965..4261332	+	1368	WP_235125544.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (40-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11205.59 Da
• Isoelectric Point: 7.5237

>T158757 WP_128612218.1 NZ_CP054129:c4257266-4256979 [Citrobacter portucalensis]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHMSRQDRYENGEYRWQTIGIVHGIVVILVAHSVRFESGVEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T158757 NZ_CP069799:c2479360-2479094 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11486.04 Da
• Isoelectric Point: 9.5646

>AT158757 WP_128612219.1 NZ_CP054129:c4256992-4256690 [Citrobacter portucalensis]
MSMVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDEQWSEANRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT158757 NZ_CP069799:c2479611-2479360 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA