Detailed information of TA system TA158728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2934497..2936096
• Replicon: chromosome
• Accession: NZ_CP054117
• Organism: Bordetella pertussis strain NZ13

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRJ67_RS14130
• Protein ID: WP_003816876.1
• Coordinates: 2934497..2935837 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRJ67_RS14135
• Protein ID: WP_019247974.1
• Coordinates: 2935839..2936096 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ67_RS14110 (HRJ67_14005)	2929723..2930526	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRJ67_RS14115 (HRJ67_14010)	2930959..2932037	-	1079	Protein_2776	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRJ67_RS14120 (HRJ67_14015)	2932269..2933699	-	1431	WP_019247976.1	MFS transporter	-
HRJ67_RS14125 (HRJ67_14020)	2933712..2934401	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRJ67_RS14130 (HRJ67_14025)	2934497..2935837	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRJ67_RS14135 (HRJ67_14030)	2935839..2936096	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRJ67_RS14140 (HRJ67_14035)	2936450..2938042	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRJ67_RS14145 (HRJ67_14040)	2938139..2939599	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRJ67_RS14150 (HRJ67_14045)	2939822..2940433	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158728 WP_003816876.1 NZ_CP054117:c2935837-2934497 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158728 NZ_CP069796:c1870538-1870071 [Actinobacillus pleuropneumoniae serovar 6 str. Femo]
ATGCACGCACCGGAACTGCTTTCCGAGCAACATATTGTGCGATATTTTCAATGTGGTGAGGTCGTGCTAGACCAATGGCT
ACAACGTACTGCATTGAAAAATCAGCAAAATAATGCCAGTAAAACCTTTGTTGTGCGTGATGAGAATAAACAGGTGATGG
GATTTTATTGTTTATCTGCCGGTTCGGTGAGCCATCAGTTTGTTGCAGGCGCATTAAGACGTAATATGCCCGATCCGATT
CCCGTGATTATTTTAGGTAGATTAGCGGTCGATTGCTCAGCACAGGGTAAGCAGTTAGGCGTATCAATGTTAAAAGATGC
GGTCTTAAAATCGAAGACGGTGGCGAACCAAATCGGGATAAAAGCATTGCTTGTTCATGCGTTAAACCCGCAAGCAAAAC
AATTTTATTTAAAGTATGGTTTTTCATGTTCACCCATTGATGAGATGGTATTAATGTTAAAACTCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158728 WP_019247974.1 NZ_CP054117:c2936096-2935839 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158728 NZ_CP069796:c1870802-1870542 [Actinobacillus pleuropneumoniae serovar 6 str. Femo]
ATGAGAACTGCGCCAATTAATTTACGAGCGATGCCTGATCAGCGTGATTTAATTGATTATGCGGCAAGCATTTTAGGTAA
AACACGTTCTGACTTTATGTTAGAAACCGCCTGCCAAGCTGCCCAAAATGTGATTTTAGATCGTACCGTTTTCCAATTAA
ATGAGCAGGCTTTCGAGCAATTTAACCGATTGCTAGAACAGCCAACAGCAGATAATCCGGGGTTAGATAAACTGTTAAAT
ACCAAAGCGGTGTGGGAGTAG

Structures

Toxin

• AlphaFold DB: Q7VVM6