Detailed information of TA system TA158725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2740976..2742575
• Replicon: chromosome
• Accession: NZ_CP054116
• Organism: Bordetella pertussis strain NZ14

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRJ68_RS13330
• Protein ID: WP_003816876.1
• Coordinates: 2740976..2742316 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRJ68_RS13335
• Protein ID: WP_019247974.1
• Coordinates: 2742318..2742575 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ68_RS13310 (HRJ68_13175)	2736202..2737005	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRJ68_RS13315 (HRJ68_13180)	2737438..2738516	-	1079	Protein_2620	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRJ68_RS13320 (HRJ68_13185)	2738748..2740178	-	1431	WP_019247976.1	MFS transporter	-
HRJ68_RS13325 (HRJ68_13190)	2740191..2740880	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRJ68_RS13330 (HRJ68_13195)	2740976..2742316	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRJ68_RS13335 (HRJ68_13200)	2742318..2742575	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRJ68_RS13340 (HRJ68_13205)	2742929..2744521	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRJ68_RS13345 (HRJ68_13210)	2744618..2746078	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRJ68_RS13350 (HRJ68_13215)	2746301..2746912	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158725 WP_003816876.1 NZ_CP054116:c2742316-2740976 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158725 NZ_CP069796:1571074-1571325 [Actinobacillus pleuropneumoniae serovar 6 str. Femo]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158725 WP_019247974.1 NZ_CP054116:c2742575-2742318 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158725 NZ_CP069796:1571322-1571582 [Actinobacillus pleuropneumoniae serovar 6 str. Femo]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Structures

Toxin

• AlphaFold DB: Q7VVM6