Detailed information of TA system TA158716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2744131..2745730
• Replicon: chromosome
• Accession: NZ_CP054109
• Organism: Bordetella pertussis strain NZ21

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRJ75_RS13270
• Protein ID: WP_003816876.1
• Coordinates: 2744131..2745471 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRJ75_RS13275
• Protein ID: WP_019247974.1
• Coordinates: 2745473..2745730 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ75_RS13250 (HRJ75_13270)	2739357..2740160	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRJ75_RS13255 (HRJ75_13275)	2740593..2741671	-	1079	Protein_2602	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRJ75_RS13260 (HRJ75_13280)	2741903..2743333	-	1431	WP_019247976.1	MFS transporter	-
HRJ75_RS13265 (HRJ75_13285)	2743346..2744035	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRJ75_RS13270 (HRJ75_13290)	2744131..2745471	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRJ75_RS13275 (HRJ75_13295)	2745473..2745730	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRJ75_RS13280 (HRJ75_13300)	2746084..2747676	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRJ75_RS13285 (HRJ75_13305)	2747773..2749233	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRJ75_RS13290 (HRJ75_13310)	2749456..2750067	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158716 WP_003816876.1 NZ_CP054109:c2745471-2744131 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158716 NZ_CP069789:513687-514037 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158716 WP_019247974.1 NZ_CP054109:c2745730-2745473 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158716 NZ_CP069789:513401-513682 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: Q7VVM6