Detailed information of TA system TA158713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2745181..2746780
• Replicon: chromosome
• Accession: NZ_CP054107
• Organism: Bordetella pertussis strain NZ23

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRJ77_RS13355
• Protein ID: WP_003816876.1
• Coordinates: 2745181..2746521 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRJ77_RS13360
• Protein ID: WP_019247974.1
• Coordinates: 2746523..2746780 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ77_RS13335 (HRJ77_13270)	2740407..2741210	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRJ77_RS13340 (HRJ77_13275)	2741643..2742721	-	1079	Protein_2627	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRJ77_RS13345 (HRJ77_13280)	2742953..2744383	-	1431	WP_019247976.1	MFS transporter	-
HRJ77_RS13350 (HRJ77_13285)	2744396..2745085	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRJ77_RS13355 (HRJ77_13290)	2745181..2746521	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRJ77_RS13360 (HRJ77_13295)	2746523..2746780	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRJ77_RS13365 (HRJ77_13300)	2747134..2748726	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRJ77_RS13370 (HRJ77_13305)	2748823..2750283	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRJ77_RS13375 (HRJ77_13310)	2750506..2751117	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158713 WP_003816876.1 NZ_CP054107:c2746521-2745181 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158713 NZ_CP069714:44793-45173 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACTGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCAACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158713 WP_019247974.1 NZ_CP054107:c2746780-2746523 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158713 NZ_CP069714:44572-44793 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: Q7VVM6