Detailed information of TA system TA158710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2746215..2747814
• Replicon: chromosome
• Accession: NZ_CP054106
• Organism: Bordetella pertussis strain NZ24

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRJ78_RS13275
• Protein ID: WP_003816876.1
• Coordinates: 2746215..2747555 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRJ78_RS13280
• Protein ID: WP_019247974.1
• Coordinates: 2747557..2747814 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ78_RS13255 (HRJ78_13280)	2741441..2742244	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRJ78_RS13260 (HRJ78_13285)	2742677..2743755	-	1079	Protein_2607	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRJ78_RS13265 (HRJ78_13290)	2743987..2745417	-	1431	WP_019247976.1	MFS transporter	-
HRJ78_RS13270 (HRJ78_13295)	2745430..2746119	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRJ78_RS13275 (HRJ78_13300)	2746215..2747555	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRJ78_RS13280 (HRJ78_13305)	2747557..2747814	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRJ78_RS13285 (HRJ78_13310)	2748168..2749760	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRJ78_RS13290 (HRJ78_13315)	2749857..2751317	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRJ78_RS13295 (HRJ78_13320)	2751540..2752151	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158710 WP_003816876.1 NZ_CP054106:c2747555-2746215 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158710 NZ_CP069713:c151187-150882 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158710 WP_019247974.1 NZ_CP054106:c2747814-2747557 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158710 NZ_CP069713:c151407-151189 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: Q7VVM6