Detailed information of TA system TA158696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1310847..1312446
• Replicon: chromosome
• Accession: NZ_CP054091
• Organism: Bordetella pertussis strain NZ40

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRJ94_RS06350
• Protein ID: WP_003816876.1
• Coordinates: 1311106..1312446 (+)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRJ94_RS06345
• Protein ID: WP_019247974.1
• Coordinates: 1310847..1311104 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRJ94_RS06330 (HRJ94_06225)	1306510..1307121	-	612	WP_010931041.1	DUF2239 family protein	-
HRJ94_RS06335 (HRJ94_06230)	1307344..1308804	+	1461	WP_003812368.1	IMP dehydrogenase	-
HRJ94_RS06340 (HRJ94_06235)	1308901..1310493	+	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRJ94_RS06345 (HRJ94_06240)	1310847..1311104	+	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRJ94_RS06350 (HRJ94_06245)	1311106..1312446	+	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRJ94_RS06355 (HRJ94_06250)	1312542..1313231	-	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRJ94_RS06360 (HRJ94_06255)	1313244..1314674	+	1431	WP_019247976.1	MFS transporter	-
HRJ94_RS06365 (HRJ94_06260)	1314906..1315984	+	1079	Protein_1257	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRJ94_RS06370 (HRJ94_06265)	1316417..1317220	+	804	WP_019248072.1	IclR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158696 WP_003816876.1 NZ_CP054091:1311106-1312446 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158696 NZ_CP069712:1829956-1830210 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCTCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158696 WP_019247974.1 NZ_CP054091:1310847-1311104 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158696 NZ_CP069712:1829708-1829959 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q7VVM6