Detailed information of TA system TA158686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2747181..2748780
• Replicon: chromosome
• Accession: NZ_CP054079
• Organism: Bordetella pertussis strain NZ52

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRK07_RS13370
• Protein ID: WP_003816876.1
• Coordinates: 2747181..2748521 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRK07_RS13375
• Protein ID: WP_019247974.1
• Coordinates: 2748523..2748780 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRK07_RS13350 (HRK07_13275)	2742407..2743210	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRK07_RS13355 (HRK07_13280)	2743643..2744721	-	1079	Protein_2630	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRK07_RS13360 (HRK07_13285)	2744953..2746383	-	1431	WP_019247976.1	MFS transporter	-
HRK07_RS13365 (HRK07_13290)	2746396..2747085	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRK07_RS13370 (HRK07_13295)	2747181..2748521	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRK07_RS13375 (HRK07_13300)	2748523..2748780	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRK07_RS13380 (HRK07_13305)	2749134..2750726	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRK07_RS13385 (HRK07_13310)	2750823..2752283	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRK07_RS13390 (HRK07_13315)	2752506..2753117	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158686 WP_003816876.1 NZ_CP054079:c2748521-2747181 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158686 NZ_CP069710:4085957-4086334 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCTGGAATTC
AGTGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGAGCTCGCCGGGCGACTGGCCTGATGACCCGCGA
CAATTACCGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158686 WP_019247974.1 NZ_CP054079:c2748780-2748523 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158686 NZ_CP069710:4085500-4085868 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACAACAACAACGATCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCACTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: Q7VVM6