Detailed information of TA system TA158683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2747280..2748879
• Replicon: chromosome
• Accession: NZ_CP054077
• Organism: Bordetella pertussis strain NZ54

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRK09_RS13370
• Protein ID: WP_003816876.1
• Coordinates: 2747280..2748620 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRK09_RS13375
• Protein ID: WP_019247974.1
• Coordinates: 2748622..2748879 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRK09_RS13350 (HRK09_13285)	2742506..2743309	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRK09_RS13355 (HRK09_13290)	2743742..2744820	-	1079	Protein_2630	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRK09_RS13360 (HRK09_13295)	2745052..2746482	-	1431	WP_019247976.1	MFS transporter	-
HRK09_RS13365 (HRK09_13300)	2746495..2747184	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRK09_RS13370 (HRK09_13305)	2747280..2748620	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRK09_RS13375 (HRK09_13310)	2748622..2748879	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRK09_RS13380 (HRK09_13315)	2749233..2750825	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRK09_RS13385 (HRK09_13320)	2750922..2752382	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRK09_RS13390 (HRK09_13325)	2752605..2753216	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158683 WP_003816876.1 NZ_CP054077:c2748620-2747280 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158683 NZ_CP069710:3454129-3454302 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158683 WP_019247974.1 NZ_CP054077:c2748879-2748622 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158683 NZ_CP069710:3453805-3454101 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: Q7VVM6