Detailed information of TA system TA158677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2745873..2747472
• Replicon: chromosome
• Accession: NZ_CP054075
• Organism: Bordetella pertussis strain NZ56

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRK11_RS13355
• Protein ID: WP_003816876.1
• Coordinates: 2745873..2747213 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRK11_RS13360
• Protein ID: WP_019247974.1
• Coordinates: 2747215..2747472 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRK11_RS13335 (HRK11_13265)	2741099..2741902	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRK11_RS13340 (HRK11_13270)	2742335..2743413	-	1079	Protein_2627	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRK11_RS13345 (HRK11_13275)	2743645..2745075	-	1431	WP_019247976.1	MFS transporter	-
HRK11_RS13350 (HRK11_13280)	2745088..2745777	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRK11_RS13355 (HRK11_13285)	2745873..2747213	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRK11_RS13360 (HRK11_13290)	2747215..2747472	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRK11_RS13365 (HRK11_13295)	2747826..2749418	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRK11_RS13370 (HRK11_13300)	2749515..2750975	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRK11_RS13375 (HRK11_13305)	2751198..2751809	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158677 WP_003816876.1 NZ_CP054075:c2747213-2745873 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158677 NZ_CP069710:c978129-978026 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158677 WP_019247974.1 NZ_CP054075:c2747472-2747215 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158677 NZ_CP069710:977988-978259 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: Q7VVM6