Detailed information of TA system TA158668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2745669..2747268
• Replicon: chromosome
• Accession: NZ_CP054071
• Organism: Bordetella pertussis strain NZ60

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRK14_RS13280
• Protein ID: WP_003816876.1
• Coordinates: 2745669..2747009 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRK14_RS13285
• Protein ID: WP_019247974.1
• Coordinates: 2747011..2747268 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRK14_RS13260 (HRK14_13195)	2740895..2741698	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRK14_RS13265 (HRK14_13200)	2742131..2743209	-	1079	Protein_2606	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRK14_RS13270 (HRK14_13205)	2743441..2744871	-	1431	WP_019247976.1	MFS transporter	-
HRK14_RS13275 (HRK14_13210)	2744884..2745573	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRK14_RS13280 (HRK14_13215)	2745669..2747009	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRK14_RS13285 (HRK14_13220)	2747011..2747268	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRK14_RS13290 (HRK14_13225)	2747622..2749214	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRK14_RS13295 (HRK14_13230)	2749311..2750771	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRK14_RS13300 (HRK14_13235)	2750994..2751605	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158668 WP_003816876.1 NZ_CP054071:c2747009-2745669 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158668 NZ_CP069709:c3817647-3817333 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158668 WP_019247974.1 NZ_CP054071:c2747268-2747011 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158668 NZ_CP069709:c3817883-3817650 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: Q7VVM6