Detailed information of TA system TA158665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2747268..2748867
• Replicon: chromosome
• Accession: NZ_CP054070
• Organism: Bordetella pertussis strain NZ61

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRK15_RS13295
• Protein ID: WP_003816876.1
• Coordinates: 2747268..2748608 (-)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRK15_RS13300
• Protein ID: WP_019247974.1
• Coordinates: 2748610..2748867 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRK15_RS13275 (HRK15_13285)	2742494..2743297	-	804	WP_019248072.1	IclR family transcriptional regulator	-
HRK15_RS13280 (HRK15_13290)	2743730..2744808	-	1079	Protein_2610	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRK15_RS13285 (HRK15_13295)	2745040..2746470	-	1431	WP_019247976.1	MFS transporter	-
HRK15_RS13290 (HRK15_13300)	2746483..2747172	+	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRK15_RS13295 (HRK15_13305)	2747268..2748608	-	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRK15_RS13300 (HRK15_13310)	2748610..2748867	-	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRK15_RS13305 (HRK15_13315)	2749221..2750813	-	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRK15_RS13310 (HRK15_13320)	2750910..2752370	-	1461	WP_003812368.1	IMP dehydrogenase	-
HRK15_RS13315 (HRK15_13325)	2752593..2753204	+	612	WP_010931041.1	DUF2239 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158665 WP_003816876.1 NZ_CP054070:c2748608-2747268 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158665 NZ_CP069709:3408759-3408977 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158665 WP_019247974.1 NZ_CP054070:c2748867-2748610 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158665 NZ_CP069709:3408359-3408733 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: Q7VVM6