Detailed information of TA system TA158660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1279699..1281298
• Replicon: chromosome
• Accession: NZ_CP054067
• Organism: Bordetella pertussis strain NZ64

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: HRK18_RS06230
• Protein ID: WP_003816876.1
• Coordinates: 1279958..1281298 (+)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRK18_RS06225
• Protein ID: WP_019247974.1
• Coordinates: 1279699..1279956 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRK18_RS06210 (HRK18_06160)	1275362..1275973	-	612	WP_010931041.1	DUF2239 family protein	-
HRK18_RS06215 (HRK18_06165)	1276196..1277656	+	1461	WP_003812368.1	IMP dehydrogenase	-
HRK18_RS06220 (HRK18_06170)	1277753..1279345	+	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
HRK18_RS06225 (HRK18_06175)	1279699..1279956	+	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
HRK18_RS06230 (HRK18_06180)	1279958..1281298	+	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRK18_RS06235 (HRK18_06185)	1281394..1282083	-	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
HRK18_RS06240 (HRK18_06190)	1282096..1283526	+	1431	WP_019247976.1	MFS transporter	-
HRK18_RS06245 (HRK18_06195)	1283758..1284836	+	1079	Protein_1232	tellurite resistance/C4-dicarboxylate transporter family protein	-
HRK18_RS06250 (HRK18_06200)	1285269..1286072	+	804	WP_019248072.1	IclR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T158660 WP_003816876.1 NZ_CP054067:1279958-1281298 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T158660 NZ_CP069709:1753698-1753952 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT158660 WP_019247974.1 NZ_CP054067:1279699-1279956 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT158660 NZ_CP069709:1753450-1753701 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: Q7VVM6