Detailed information of TA system TA158656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 196226..196896
• Replicon: plasmid pSWHvKp
• Accession: NZ_CP054064
• Organism: Klebsiella pneumoniae strain WSHvKP

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HQ868_RS25815
• Protein ID: WP_004213072.1
• Coordinates: 196453..196896 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HQ868_RS25810
• Protein ID: WP_004213073.1
• Coordinates: 196226..196456 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ868_RS25785	192449..193348	-	900	WP_004225022.1	nucleotide-binding protein	-
HQ868_RS25790	193338..193628	-	291	WP_004213078.1	hypothetical protein	-
HQ868_RS25795	193980..194186	+	207	WP_004213077.1	hypothetical protein	-
HQ868_RS25800	194176..194469	-	294	WP_004213076.1	hypothetical protein	-
HQ868_RS25805	194485..195618	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HQ868_RS25810	196226..196456	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HQ868_RS25815	196453..196896	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HQ868_RS25820	196910..197326	-	417	Protein_208	restriction endonuclease	-
HQ868_RS25825	197330..197623	-	294	Protein_209	transposase	-
HQ868_RS25830	198040..198679	+	640	Protein_210	response regulator	-
HQ868_RS25835	199195..199601	-	407	Protein_211	GAF domain-containing protein	-
HQ868_RS25840	199692..200612	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
HQ868_RS25845	200661..201152	-	492	WP_011251327.1	DM13 domain-containing protein	-
HQ868_RS25850	201215..201490	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..211588	211588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T158656 WP_004213072.1 NZ_CP054064:196453-196896 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T158656 NZ_CP069709:694766-695143 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT158656 WP_004213073.1 NZ_CP054064:196226-196456 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT158656 NZ_CP069709:694308-694676 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATTCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620