Detailed information of TA system TA158653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 50054..50790
• Replicon: plasmid pSWHvKp
• Accession: NZ_CP054064
• Organism: Klebsiella pneumoniae strain WSHvKP

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HQ868_RS25035
• Protein ID: WP_117985291.1
• Coordinates: 50308..50790 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: HQ868_RS25030
• Protein ID: WP_004213599.1
• Coordinates: 50054..50320 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ868_RS25005	45286..45843	+	558	WP_004213590.1	recombinase family protein	-
HQ868_RS25010	45846..48815	+	2970	WP_094936121.1	Tn3 family transposase	-
HQ868_RS25015	48857..49351	+	495	WP_004213594.1	hypothetical protein	-
HQ868_RS25020	49412..49615	+	204	WP_004213596.1	hemolysin expression modulator Hha	-
HQ868_RS25025	49629..49859	+	231	WP_004213598.1	hypothetical protein	-
HQ868_RS25030	50054..50320	+	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
HQ868_RS25035	50308..50790	+	483	WP_117985291.1	GNAT family N-acetyltransferase	Toxin
HQ868_RS25040	51211..52438	+	1228	Protein_52	IS3 family transposase	-
HQ868_RS25045	52434..52829	-	396	Protein_53	DDE-type integrase/transposase/recombinase	-
HQ868_RS25050	53004..54026	-	1023	WP_004214536.1	porphobilinogen synthase	-
HQ868_RS25055	54090..55436	-	1347	WP_048333011.1	dihydroorotase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..211588	211588
	-	inside	IScluster/Tn	-	-	45286..52438	7152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T158653 WP_117985291.1 NZ_CP054064:50308-50790 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENLGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T158653 NZ_CP069708:c54321-54040 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT158653 WP_004213599.1 NZ_CP054064:50054-50320 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT158653 NZ_CP069708:c54562-54311 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A7D3T1D0