Detailed information of TA system TA158650

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4478349..4479040
• Replicon: chromosome
• Accession: NZ_CP054063
• Organism: Klebsiella pneumoniae strain WSHvKP

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HQ868_RS21785
• Protein ID: WP_129073948.1
• Coordinates: 4478349..4478690 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2A5MHA6
• Locus tag: HQ868_RS21790
• Protein ID: WP_019725272.1
• Coordinates: 4478714..4479040 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ868_RS21750	4473572..4473802	-	231	WP_077256410.1	hypothetical protein	-
HQ868_RS21755	4473871..4474515	-	645	WP_164972967.1	hypothetical protein	-
HQ868_RS21760	4474512..4475204	-	693	WP_040173594.1	HNH endonuclease	-
HQ868_RS21765	4475401..4475983	+	583	Protein_4265	tyrosine-type recombinase/integrase	-
HQ868_RS21770	4476124..4477032	+	909	WP_077256408.1	hypothetical protein	-
HQ868_RS21775	4477111..4477395	+	285	Protein_4267	helix-turn-helix domain-containing protein	-
HQ868_RS21780	4477401..4478234	-	834	WP_040173641.1	DUF4942 domain-containing protein	-
HQ868_RS21785	4478349..4478690	-	342	WP_129073948.1	TA system toxin CbtA family protein	Toxin
HQ868_RS21790	4478714..4479040	-	327	WP_019725272.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HQ868_RS21795	4479054..4479530	-	477	WP_019725273.1	DNA repair protein RadC	-
HQ868_RS21800	4479540..4479986	-	447	WP_040173599.1	antirestriction protein	-
HQ868_RS21805	4480197..4480886	-	690	WP_009309812.1	hypothetical protein	-
HQ868_RS21810	4481451..4482341	-	891	WP_085163516.1	50S ribosome-binding GTPase	-
HQ868_RS21815	4482424..4483512	-	1089	WP_085163515.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4460809..4500204	39395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12885.93 Da
• Isoelectric Point: 8.5195

>T158650 WP_129073948.1 NZ_CP054063:c4478690-4478349 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTMNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Nucleotide

• Length: 342 bp

>T158650 NZ_CP069707:3611323-3611541 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12276.01 Da
• Isoelectric Point: 6.9560

>AT158650 WP_019725272.1 NZ_CP054063:c4479040-4478714 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT158650 NZ_CP069707:3610923-3611297 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2A5MHA6