Detailed information of TA system TA158649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4433001..4433811
• Replicon: chromosome
• Accession: NZ_CP054063
• Organism: Klebsiella pneumoniae strain WSHvKP

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HQ868_RS21555
• Protein ID: WP_173424252.1
• Coordinates: 4433001..4433534 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HQ868_RS21560
• Protein ID: WP_002887278.1
• Coordinates: 4433545..4433811 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ868_RS21550	4431832..4432953	+	1122	WP_101971391.1	cupin domain-containing protein	-
HQ868_RS21555	4433001..4433534	-	534	WP_173424252.1	GNAT family N-acetyltransferase	Toxin
HQ868_RS21560	4433545..4433811	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HQ868_RS21565	4433914..4435347	-	1434	WP_129073943.1	Cu(+)/Ag(+) sensor histidine kinase	-
HQ868_RS21570	4435337..4436020	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HQ868_RS21575	4436193..4437578	+	1386	WP_029602902.1	efflux transporter outer membrane subunit	-
HQ868_RS21580	4437596..4437901	+	306	WP_032432083.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19814.64 Da
• Isoelectric Point: 5.2614

>T158649 WP_173424252.1 NZ_CP054063:c4433534-4433001 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKITYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T158649 NZ_CP069707:3027932-3028210 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGCTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT158649 WP_002887278.1 NZ_CP054063:c4433811-4433545 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT158649 NZ_CP069707:3027739-3027930 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1