Detailed information of TA system TA158635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3296919..3298639
• Replicon: chromosome
• Accession: NZ_CP054060
• Organism: Edwardsiella ictaluri strain T1-1

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: F8539_RS15580
• Protein ID: WP_198077154.1
• Coordinates: 3297983..3298639 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: F8539_RS15575
• Protein ID: WP_012849888.1
• Coordinates: 3296919..3297986 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F8539_RS15560	3292159..3292713	+	555	WP_049640356.1	hypothetical protein	-
F8539_RS15565	3292944..3293660	-	717	WP_198077153.1	M48 family metallopeptidase	-
F8539_RS15570	3293660..3296755	-	3096	WP_049640354.1	HsdR family type I site-specific deoxyribonuclease	-
F8539_RS15575	3296919..3297986	-	1068	WP_012849888.1	macro domain-containing protein	Antitoxin
F8539_RS15580	3297983..3298639	-	657	WP_198077154.1	DUF4433 domain-containing protein	Toxin
F8539_RS15585	3298652..3300028	-	1377	WP_198077391.1	restriction endonuclease subunit S	-
F8539_RS15590	3300040..3302478	-	2439	WP_198077155.1	type I restriction-modification system subunit M	-
F8539_RS15600	3303385..3303624	-	240	WP_015872655.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25210.74 Da
• Isoelectric Point: 6.9049

>T158635 WP_198077154.1 NZ_CP054060:c3298639-3297983 [Edwardsiella ictaluri]
MAYDYSANLNPEKALIWRIVHRDNIPWILDNGLHCGNSQVQAANWINIGNPELIGKRAGHPVPVGMGGTLHDYVPFYFTP
FSPMLMNIHSGRGGITQRPNEEIVILVSNLRNVAAQNIPFVFTDSHAYYNWVNYYTRLEDLNQIDWPILQARDFRRDPDD
PAKFERYQAEALIWKHCPITLLVGMICYNDGVRLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T158635 NZ_CP069706:c4177593-4177243 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40713.02 Da
• Isoelectric Point: 6.9067

>AT158635 WP_012849888.1 NZ_CP054060:c3297986-3296919 [Edwardsiella ictaluri]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKAYAHACKQKQVMTGKMFITETGELMGPRWIVNFPTK
QHWRADSRMAWIEDGLQDLRRFLIEKKVRSIAIPPLGAGNGGLNWPDVRARIESTLGDLQDTEILIYEPTEKYQNVAKSS
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQEDVLKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHINLWLNSEAKAWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGQCQPTLDSVKEG
LHQWPAGEPWASRKIKLFDDKNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT158635 NZ_CP069706:c4177838-4177587 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA