Detailed information of TA system TA158585

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 1686056..1686756
• Replicon: chromosome
• Accession: NZ_CP054049
• Organism: Yersinia kristensenii strain 2012N-4030

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HRD70_RS07485
• Protein ID: WP_098905178.1
• Coordinates: 1686056..1686427 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HRD70_RS07490
• Protein ID: WP_098905179.1
• Coordinates: 1686424..1686756 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRD70_RS07455	1681882..1682319	+	438	WP_172986259.1	hypothetical protein	-
HRD70_RS07460	1682339..1683608	+	1270	Protein_1439	hypothetical protein	-
HRD70_RS07465	1683622..1683795	+	174	WP_172668036.1	hypothetical protein	-
HRD70_RS07470	1683831..1684268	+	438	WP_050290232.1	hypothetical protein	-
HRD70_RS07475	1684351..1685172	+	822	WP_172986260.1	DUF945 domain-containing protein	-
HRD70_RS07480	1685533..1685964	+	432	WP_098905177.1	antirestriction protein	-
HRD70_RS07485	1686056..1686427	+	372	WP_098905178.1	TA system toxin CbtA family protein	Toxin
HRD70_RS07490	1686424..1686756	+	333	WP_098905179.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HRD70_RS07495	1686841..1687701	+	861	WP_098905180.1	DUF4942 domain-containing protein	-
HRD70_RS07500	1688124..1689170	+	1047	WP_050094957.1	ParB/Srx family N-terminal domain-containing protein	-
HRD70_RS07505	1689246..1690142	-	897	WP_049558050.1	LysR family transcriptional regulator	-
HRD70_RS07510	1690317..1691327	+	1011	WP_050094956.1	NAD(P)-dependent alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (13-101)

Antitoxin

• Antitoxin CbeA (9-108)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13624.67 Da
• Isoelectric Point: 6.8773

>T158585 WP_098905178.1 NZ_CP054049:1686056-1686427 [Yersinia kristensenii]
MKPNPTTAGALPVPVIYQQLLAFLLEHHYGLSLNDTPYHDEQVIAQQIEQGISVGDTINTLVEKYALVRIGRNGFSALAQ
DPMLSKGDIVRARQAVGLWRAHFPPTLLYPTESLNPIPQRPLT

Nucleotide

• Length: 372 bp

>T158585 NZ_CP069692:c612918-612454 [Escherichia coli H20]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12091.72 Da
• Isoelectric Point: 6.9660

>AT158585 WP_098905179.1 NZ_CP054049:1686424-1686756 [Yersinia kristensenii]
MNNHITTSPTWGLKRDITPRLGARLVQEGTHLHFLSDRANIAGTFSLEATQALDAAFPALITKLESQLLSGQLDPRRQQC
LTVRHGGFVCEADTLGSHGYVYIAVYQELA

Nucleotide

• Length: 333 bp

>AT158585 NZ_CP069692:c613253-612918 [Escherichia coli H20]
ATGCCCGCTAATGCTCGCTCTCACGCAGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
TCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACGATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
TCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA