Detailed information of TA system TA158567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1227165..1228715
• Replicon: chromosome
• Accession: NZ_CP054048
• Organism: Yersinia massiliensis strain 2011N-4075

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0T9TNV2
• Locus tag: HRD68_RS06490
• Protein ID: WP_050080816.1
• Coordinates: 1227165..1228460 (-)
• Length: 432 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HRD68_RS06495
• Protein ID: WP_050080817.1
• Coordinates: 1228464..1228715 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRD68_RS06465	1222935..1223936	+	1002	WP_108088085.1	autoinducer 2 ABC transporter permease LsrD	-
HRD68_RS06470	1224017..1225036	+	1020	WP_019211214.1	autoinducer 2 ABC transporter substrate-binding protein LsrB	-
HRD68_RS06475	1225092..1225967	+	876	WP_019211215.1	3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase	-
HRD68_RS06480	1225988..1226278	+	291	WP_019211216.1	(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase	-
HRD68_RS06485	1226519..1227001	+	483	WP_050080815.1	DUF2501 domain-containing protein	-
HRD68_RS06490	1227165..1228460	-	1296	WP_050080816.1	type II toxin-antitoxin system HipA family toxin	Toxin
HRD68_RS06495	1228464..1228715	-	252	WP_050080817.1	helix-turn-helix domain-containing protein	Antitoxin
HRD68_RS06500	1228832..1229668	+	837	WP_050286206.1	3'-kinase	-
HRD68_RS06505	1229753..1231489	+	1737	WP_050080819.1	SulP family inorganic anion transporter	-
HRD68_RS06510	1231910..1232515	+	606	WP_050872449.1	response regulator transcription factor	-
HRD68_RS06515	1232714..1233310	-	597	WP_050080822.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 432 a.a.
• Molecular weight: 48487.86 Da
• Isoelectric Point: 6.5940

>T158567 WP_050080816.1 NZ_CP054048:c1228460-1227165 [Yersinia massiliensis]
MEKLTLALNGIVVGTLEKAAGGEISFFYHDSWLERSGARAISLSLPLQTVKFRGEVVYNFFDNLLPDSESIRSRIQARFQ
TPTKQPFDLLSQIGSDCVGAIQLYPEGRQINSVTEIHATPLNDNEIEKLLLGYKEAPLGMEADIDDFRISLAGAQEKTAL
LWYQNQWHRPEGSTPTSHILKLPIGYIAANGIDLSESVENEWLCLKIAEEFGLPVAYSEIKYFGKQKALVVERFDRRWSP
NNSWLMRLPQEDFCQVLGVAPALKYESDGGPGIADAMKLLLGSQQAIQDRDIFFKSQILFWMLAAIDGHAKNFSVFIELD
SAFRMTPIYDVMSAFPLMHPRGIPAKKAKMAMALLGRNRHFHWSTILPRHFISTAKQVNYPEDNVVQLMAEMKEKTEQVI
AAVKAVLPQGFPEKISEPIFSGLRRQSAKLP

Nucleotide

• Length: 1296 bp

>T158567 NZ_CP069677:c2441541-2440219 [Escherichia coli O126:H45]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9584.94 Da
• Isoelectric Point: 10.4866

>AT158567 WP_050080817.1 NZ_CP054048:c1228715-1228464 [Yersinia massiliensis]
MKITSAAMLANTLRNERKKSKLTQRKTAETVGLKQSTVSEFENHPDGTKLDTLFKLLAALDLELQVRPRNRNIKDNTTWD
QEW

Nucleotide

• Length: 252 bp

>AT158567 NZ_CP069677:c2441807-2441541 [Escherichia coli O126:H45]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0T9TNV2