Detailed information of TA system TA158564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 68315..69351
• Replicon: plasmid unnamed1
• Accession: NZ_CP054046
• Organism: Yersinia massiliensis strain 2011N-4075

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HRD68_RS00375
• Protein ID: WP_042807110.1
• Coordinates: 68776..69351 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: HRD68_RS00370
• Protein ID: WP_019213419.1
• Coordinates: 68315..68776 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRD68_RS00330	63609..63707	-	99	Protein_65	IS6 family transposase	-
HRD68_RS00335	63738..64187	+	450	WP_019213425.1	type II toxin-antitoxin system HicB family antitoxin	-
HRD68_RS00340	64406..65101	+	696	WP_019213424.1	IS6 family transposase	-
HRD68_RS00345	65178..65894	-	717	WP_081578887.1	tyrosine-type recombinase/integrase	-
HRD68_RS00350	66373..66690	-	318	WP_019213422.1	CcdB family protein	-
HRD68_RS00355	66690..66935	-	246	WP_019213421.1	type II toxin-antitoxin system CcdA family antitoxin	-
HRD68_RS00360	67229..67363	-	135	Protein_71	IS6 family transposase	-
HRD68_RS00365	67416..68111	-	696	WP_019213420.1	IS6 family transposase	-
HRD68_RS00370	68315..68776	+	462	WP_019213419.1	helix-turn-helix domain-containing protein	Antitoxin
HRD68_RS00375	68776..69351	+	576	WP_042807110.1	PIN domain-containing protein	Toxin
HRD68_RS00380	69662..70630	+	969	WP_172986740.1	recombinase family protein	-
HRD68_RS00385	70668..71000	-	333	WP_019213416.1	hypothetical protein	-
HRD68_RS00390	70997..71704	-	708	WP_019213415.1	ParA family protein	-
HRD68_RS00395	72132..73388	-	1257	WP_019213414.1	translesion error-prone DNA polymerase V subunit UmuC	-
HRD68_RS00400	73396..73815	-	420	WP_019213413.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..124188	124188
	-	inside	IScluster/Tn	-	-	48476..68111	19635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21876.33 Da
• Isoelectric Point: 4.8556

>T158564 WP_042807110.1 NZ_CP054046:68776-69351 [Yersinia massiliensis]
MTHSPYPVVLDACVLYPSLLRDLLMHLGLTGLYQPKWSTIIEDEWQRNLIKNRPDLKPDQIKRTGELMNQALPDAMVIGF
EPLVGSIELPDVDDRHVVATAIFSNAEIIVTFNLKDFPQTYLDNFFIEAIHPDDFITDLFDLNQALVLSAVNKQRRIMTK
PPKSVDEYLDTLLRQGLPQTVKELSKFRSLI

Nucleotide

• Length: 576 bp

>T158564 NZ_CP069677:1924941-1925195 [Escherichia coli O126:H45]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17050.41 Da
• Isoelectric Point: 6.2229

>AT158564 WP_019213419.1 NZ_CP054046:68315-68776 [Yersinia massiliensis]
MTNSILDSLSLPAQGEIEAAVRGQRELAAYLSTKMETQKISIQDADNNTHQIELPTSSLTLLMSILGELAVGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLENGKLPFHKTGRHRRVLFADLMRYKDQRDNESSRAMLELADHSQELGFY

Nucleotide

• Length: 462 bp

>AT158564 NZ_CP069677:1924693-1924944 [Escherichia coli O126:H45]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA