Detailed information of TA system TA158561

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yfjZ/CbtA-CbeA
• Location: 4586021..4586828
• Replicon: chromosome
• Accession: NZ_CP054043
• Organism: Yersinia mollaretii ATCC 43969

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7D4PU27
• Locus tag: HRD69_RS20375
• Protein ID: WP_004874578.1
• Coordinates: 4586021..4586356 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A7D4PUK3
• Locus tag: HRD69_RS20380
• Protein ID: WP_032813883.1
• Coordinates: 4586436..4586828 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRD69_RS20335	4581358..4582059	+	702	WP_004874586.1	WYL domain-containing protein	-
HRD69_RS20340	4582065..4582712	+	648	WP_032813889.1	hypothetical protein	-
HRD69_RS20345	4583086..4583553	+	468	WP_172984636.1	hypothetical protein	-
HRD69_RS20350	4583785..4584231	+	447	WP_032813887.1	antirestriction protein	-
HRD69_RS20355	4584242..4584727	+	486	WP_004874582.1	DNA repair protein RadC	-
HRD69_RS20360	4584776..4585153	+	378	WP_004874581.1	hypothetical protein	-
HRD69_RS20365	4585153..4585470	+	318	WP_050413185.1	hypothetical protein	-
HRD69_RS20370	4585526..4586008	+	483	WP_032813885.1	hypothetical protein	-
HRD69_RS20375	4586021..4586356	+	336	WP_004874578.1	TA system toxin CbtA family protein	Toxin
HRD69_RS20380	4586436..4586828	+	393	WP_032813883.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HRD69_RS20385	4586904..4587761	+	858	WP_004874576.1	DUF4942 domain-containing protein	-
HRD69_RS20390	4588148..4588665	+	518	Protein_3964	integrase arm-type DNA-binding domain-containing protein	-
HRD69_RS20395	4588916..4589698	-	783	WP_032813881.1	hypothetical protein	-
HRD69_RS20400	4589865..4590053	+	189	WP_004874574.1	hypothetical protein	-
HRD69_RS20405	4590082..4590843	-	762	WP_004874573.1	chitin disaccharide deacetylase	-
HRD69_RS20410	4590891..4591751	-	861	WP_071984829.1	transcriptional regulator ChbR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4568126..4589779	21653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (13-103)

Antitoxin

• Antitoxin CbeA (24-122)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12543.28 Da
• Isoelectric Point: 6.2241

>T158561 WP_004874578.1 NZ_CP054043:4586021-4586356 [Yersinia mollaretii ATCC 43969]
MKTLDKIPRITPSANQARTEYQQLLTWLLAHCYGLELNDTPYHDEEAIAQQIEHGVTVRETVNELVEKFDLVRIDRSGFS
LMAQDPTLNGGDLLRARSALGLRSPAIRRLA

Nucleotide

• Length: 336 bp

>T158561 NZ_CP069677:1100870-1101205 [Escherichia coli O126:H45]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14391.08 Da
• Isoelectric Point: 6.5781

>AT158561 WP_032813883.1 NZ_CP054043:4586436-4586828 [Yersinia mollaretii ATCC 43969]
MKDNVPDTDNHTTPPHEDDIPSPVWGLQRDITPRLGARLVQQGNHLHFLSDRAGFIGTFSPDTVQALEVTFSALLTKLEA
QLQSGALDPRRQHCVTVQHAGFTCEADTLGSFGHVYIAVYQTTHPTRHKP

Nucleotide

• Length: 393 bp

>AT158561 NZ_CP069677:1100622-1100870 [Escherichia coli O126:H45]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7D4PU27

Antitoxin

• AlphaFold DB: A0A7D4PUK3