Detailed information of TA system TA158539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 554615..555171
• Replicon: plasmid pPR13E02
• Accession: NZ_CP054033
• Organism: Rhizobium indicum strain JKLM 13E

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FE844_RS32215
• Protein ID: WP_138331209.1
• Coordinates: 554926..555171 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FE844_RS32210
• Protein ID: WP_138331208.1
• Coordinates: 554615..554929 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE844_RS32190	550136..551134	+	999	WP_138331205.1	ABC transporter substrate-binding protein	-
FE844_RS32195	551142..552914	+	1773	WP_138331637.1	HAMP domain-containing protein	-
FE844_RS32200	553204..553395	-	192	WP_138331206.1	CsbD family protein	-
FE844_RS32205	553481..554227	+	747	WP_138331207.1	AprI/Inh family metalloprotease inhibitor	-
FE844_RS32210	554615..554929	-	315	WP_138331208.1	BrnA antitoxin family protein	Antitoxin
FE844_RS32215	554926..555171	-	246	WP_138331209.1	BrnT family toxin	Toxin
FE844_RS32220	555661..556257	-	597	WP_138331210.1	SOS response-associated peptidase	-
FE844_RS32225	556286..557116	-	831	WP_138331211.1	Ku protein	-
FE844_RS32230	557126..558019	-	894	WP_173863102.1	Ku protein	-
FE844_RS32235	558775..559089	+	315	WP_033183755.1	EamA family transporter	-
FE844_RS32240	559238..559987	+	750	WP_138331212.1	sulfite exporter TauE/SafE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..575483	575483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 8796.40 Da
• Isoelectric Point: 10.4806

>T158539 WP_138331209.1 NZ_CP054033:c555171-554926 [Rhizobium indicum]
MKITFDEAKRRTNIEKHGLDFADLDLLFFAGSVVVPAKGGRLMAIGAFLDGVIAVVFVTLGSEAISVISMRKASRKERSI
L

Nucleotide

• Length: 246 bp

>T158539 NZ_CP069666:2574394-2574501 [Escherichia coli O89m:H9]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11325.87 Da
• Isoelectric Point: 5.2588

>AT158539 WP_138331208.1 NZ_CP054033:c554929-554615 [Rhizobium indicum]
MTRIADSVMKISDAEEAKIQRQIASDPDAPEATDEQLAKAKPFAEALPELAESIRKNLGGRPKSDNPKVAVSIRLDPEVV
DAFKAKGEGWQSRINETLRKAVGL

Nucleotide

• Length: 315 bp

>AT158539 NZ_CP069666:c2574347-2574281 [Escherichia coli O89m:H9]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT