Detailed information of TA system TA158537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 642555..643225
• Replicon: plasmid pPR13E01
• Accession: NZ_CP054032
• Organism: Rhizobium indicum strain JKLM 13E

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FE844_RS26950
• Protein ID: WP_138331080.1
• Coordinates: 642555..642974 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J0BDK5
• Locus tag: FE844_RS26955
• Protein ID: WP_003550301.1
• Coordinates: 642971..643225 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE844_RS26925	637758..638996	-	1239	WP_138331084.1	sugar ABC transporter substrate-binding protein	-
FE844_RS26930	639102..639797	-	696	WP_138331083.1	GntR family transcriptional regulator	-
FE844_RS26935	639859..641007	+	1149	WP_173863036.1	mandelate racemase/muconate lactonizing enzyme family protein	-
FE844_RS26940	641420..641971	+	552	WP_138331081.1	DUF2087 domain-containing protein	-
FE844_RS26945	642282..642508	-	227	Protein_602	choline/ethanolamine kinase--aminoglycoside phosphotransferase	-
FE844_RS26950	642555..642974	-	420	WP_138331080.1	type II toxin-antitoxin system VapC family toxin	Toxin
FE844_RS26955	642971..643225	-	255	WP_003550301.1	plasmid stabilization protein	Antitoxin
FE844_RS26960	643487..643948	-	462	WP_138331079.1	tryptophan-rich sensory protein	-
FE844_RS26965	644177..647029	-	2853	WP_138331078.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1207524	1207524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14986.42 Da
• Isoelectric Point: 5.1532

>T158537 WP_138331080.1 NZ_CP054032:c642974-642555 [Rhizobium indicum]
MILLDTNVISEPWKPVPDGAVVAWLDAQAIETLFVSAITIAELRFGIAAMPSGRRQTILRDRLEDEVLPHFAERILPFGL
AASQFYAELMARARVSGKAIGKADGYIAATALANGLAVATRDTSPFEAVGLKVINPWLP

Nucleotide

• Length: 420 bp

>T158537 NZ_CP069666:2281403-2282725 [Escherichia coli O89m:H9]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9257.39 Da
• Isoelectric Point: 6.2260

>AT158537 WP_003550301.1 NZ_CP054032:c643225-642971 [Rhizobium indicum]
MPAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDIIEAAVRPSERVKLGSLLAAIGRDAELSNSDVEALQQNRDKAPAEP
MTFE

Nucleotide

• Length: 255 bp

>AT158537 NZ_CP069666:2281137-2281403 [Escherichia coli O89m:H9]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A6I2B3