Detailed information of TA system TA158534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 73558..74234
• Replicon: plasmid pPR13E01
• Accession: NZ_CP054032
• Organism: Rhizobium indicum strain JKLM 13E

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FE844_RS24295
• Protein ID: WP_138332918.1
• Coordinates: 73558..73983 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FE844_RS24300
• Protein ID: WP_138332916.1
• Coordinates: 73980..74234 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE844_RS24260	68813..69070	+	258	WP_003550229.1	hypothetical protein	-
FE844_RS24265	69070..69441	+	372	WP_138332930.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FE844_RS24270	69710..70435	-	726	WP_138332928.1	amino acid ABC transporter ATP-binding protein	-
FE844_RS24275	70413..71072	-	660	WP_138332926.1	amino acid ABC transporter permease	-
FE844_RS24280	71069..71740	-	672	WP_138332924.1	amino acid ABC transporter permease	-
FE844_RS24285	71822..72607	-	786	WP_138332922.1	transporter substrate-binding domain-containing protein	-
FE844_RS24290	72601..73362	-	762	WP_138332920.1	GntR family transcriptional regulator	-
FE844_RS24295	73558..73983	-	426	WP_138332918.1	type II toxin-antitoxin system VapC family toxin	Toxin
FE844_RS24300	73980..74234	-	255	WP_138332916.1	plasmid stabilization protein	Antitoxin
FE844_RS24305	74385..75155	-	771	WP_138332914.1	amino acid ABC transporter ATP-binding protein	-
FE844_RS24310	75168..75827	-	660	WP_027664855.1	amino acid ABC transporter permease	-
FE844_RS24315	75833..76495	-	663	WP_025397316.1	amino acid ABC transporter permease	-
FE844_RS24320	76613..77452	-	840	WP_138332912.1	transporter substrate-binding domain-containing protein	-
FE844_RS24325	77710..78351	-	642	WP_138332910.1	TetR/AcrR family transcriptional regulator	-
FE844_RS24330	78389..79180	+	792	WP_138332908.1	crotonase/enoyl-CoA hydratase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1207524	1207524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 14945.13 Da
• Isoelectric Point: 4.4617

>T158534 WP_138332918.1 NZ_CP054032:c73983-73558 [Rhizobium indicum]
MIILDTNVVSEAMKPAPDEAVKFWLDEQAAETLFLSSVTIAELMFGIGALSAGKRKERLCDALDGLMELFESRILAFDIT
AARRYADLAVKARAAGRGFPTPDGYIAAIAASKGFAVATRDTSAFDAAGVEVINPWAASTA

Nucleotide

• Length: 426 bp

>T158534 NZ_CP069666:1761739-1761990 [Escherichia coli O89m:H9]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9377.70 Da
• Isoelectric Point: 10.6531

>AT158534 WP_138332916.1 NZ_CP054032:c74234-73980 [Rhizobium indicum]
MPAVTIRNLSDATHRALKVRAAHHGRSAEAEMREILEAAVRPETRLRLGSALAERSRRLGLTNEDFTVLDQARDTLPAKP
MSFE

Nucleotide

• Length: 255 bp

>AT158534 NZ_CP069666:1761987-1762241 [Escherichia coli O89m:H9]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA