Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 201422..202107 Replicon plasmid pPR19E01
Accession NZ_CP054028
Organism Rhizobium hidalgonense strain JKLM 19E

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag FFM81_RS22270 Protein ID WP_128397892.1
Coordinates 201422..201844 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag FFM81_RS22275 Protein ID WP_138384579.1
Coordinates 201841..202107 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFM81_RS22250 197374..198726 + 1353 WP_138384580.1 LLM class flavin-dependent oxidoreductase -
FFM81_RS22255 198737..199588 + 852 WP_025417900.1 ABC transporter permease -
FFM81_RS22260 199627..200610 + 984 WP_025417899.1 ABC transporter substrate-binding protein -
FFM81_RS22265 200620..201390 + 771 WP_025417898.1 ABC transporter ATP-binding protein -
FFM81_RS22270 201422..201844 - 423 WP_128397892.1 type II toxin-antitoxin system VapC family toxin Toxin
FFM81_RS22275 201841..202107 - 267 WP_138384579.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FFM81_RS22280 202251..204062 - 1812 WP_138384578.1 chloride channel protein -
FFM81_RS22285 204324..204740 + 417 WP_173883875.1 MarR family transcriptional regulator -
FFM81_RS22290 204757..205665 - 909 WP_138384577.1 homocysteine S-methyltransferase family protein -
FFM81_RS22295 205784..206473 - 690 WP_138384576.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB / htpB / gmd 1..1610741 1610741


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-63)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15474.62 Da        Isoelectric Point: 4.6850

>T158529 WP_128397892.1 NZ_CP054028:c201844-201422 [Rhizobium hidalgonense]
VKLLLDTDVLSEVTKPNPDARVLEWLDKLDEDRSFISIVSIAEIRRGVALMDRGRKRDALAEWLAQDLTQRFEHRIIPVD
EPVALAWGDLMGHAKRSGRGLSSMDGLIAATAVAHDLGLATRNTSDFEGFGIELIDPWAG

Download         Length: 423 bp

>T158529 NZ_CP069666:c603110-602646 [Escherichia coli O89m:H9]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9895.12 Da        Isoelectric Point: 8.6587

>AT158529 WP_138384579.1 NZ_CP054028:c202107-201841 [Rhizobium hidalgonense]
MRPSRRGDESWTVASAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRRTARKGTLAEFLMESPLRGADLDLERQY
DEPRDPAL

Download         Length: 267 bp

>AT158529 NZ_CP069666:c603445-603110 [Escherichia coli O89m:H9]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References