Detailed information of TA system TA158500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 2591353..2592029
• Replicon: chromosome
• Accession: NZ_CP054014
• Organism: Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FOC64_RS12450
• Protein ID: WP_003898500.1
• Coordinates: 2591616..2592029 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FOC64_RS12445
• Protein ID: WP_003402915.1
• Coordinates: 2591353..2591619 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC64_RS12430	2586787..2587767	-	981	WP_003402923.1	o-succinylbenzoate synthase	-
FOC64_RS12435	2587764..2589368	-	1605	WP_003402920.1	amidohydrolase	-
FOC64_RS12440	2589446..2591161	+	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
FOC64_RS12445	2591353..2591619	+	267	WP_003402915.1	antitoxin VapB3	Antitoxin
FOC64_RS12450	2591616..2592029	+	414	WP_003898500.1	PIN domain-containing protein	Toxin
FOC64_RS12455	2592343..2593245	+	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FOC64_RS12460	2593341..2594225	+	885	WP_003402909.1	SDR family oxidoreductase	-
FOC64_RS12465	2594288..2594674	+	387	WP_003402907.1	VOC family protein	-
FOC64_RS12470	2594794..2596047	+	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
FOC64_RS12475	2596044..2596322	+	279	WP_003402901.1	hypothetical protein	-
FOC64_RS12480	2596382..2596684	+	303	WP_003402896.1	DUF3349 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T158500 WP_003898500.1 NZ_CP054014:2591616-2592029 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T158500 NZ_CP069647:c59989-59807 [Escherichia coli O7:H4]
GTGAAGTATTCAGAGTTCAAACGCTGGTTAATCCAGCAAGGGGCAGAGTTCAGGAAAGCGCCTGGTGGCGGCAGTCATCA
GAAAGTGAACCTGAACGGAAAGCGGTCGGTTTTCCCGGACCACGGTTCAAAGGAAATGCCTGAACCGCTACGCAAGAAAA
TAATGAAGGACCTGGGGCTTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT158500 WP_003402915.1 NZ_CP054014:2591353-2591619 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT158500 NZ_CP069647:c59776-59345 [Escherichia coli O7:H4]
ATGTTTAACTATCCGGTAAAACTGGAGCGCGACGACAAAACCGGCGCGTATGTCGTATCATGCCGCGACCTACCATTGTT
TAATTCCGTGGGTGACTCAGTTGAAGAAGCGTTGCTTGAGGCTGGTTATGGATTGGTCGCAGCGGTTGCGATTGAGATTG
AAGAACGCCGACCTGTTCCCACTGGTAGCGAGCCAAAAGAAGGTGAGTACGTTGTCTCTTTGCCAGTGCTACCAGCAATG
AAGGCTGCACTCCACAACGCGATGATAGAAACCGGCACACGCAAAGCAGAGTTGGCGCGCAAACTCGGAAAAAATGGAAC
ACAGATTGATCGGCTTCTCGACGTCGAACATTCATCGAAAGTTGAAACTGTCGAGCTGGCACTGCATCAACTGAATAAAA
ATATCGTCGTATCTGTCACACCAATGCGGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0