Detailed information of TA system TA158497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 2534460..2535106
• Replicon: chromosome
• Accession: NZ_CP054014
• Organism: Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: FOC64_RS12200
• Protein ID: WP_003403137.1
• Coordinates: 2534693..2535106 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: FOC64_RS12195
• Protein ID: WP_003403139.1
• Coordinates: 2534460..2534696 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC64_RS12160	2529534..2530244	-	711	Protein_2398	transposase	-
FOC64_RS12165	2530246..2530830	-	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FOC64_RS12170	2531071..2532021	-	951	WP_003900184.1	DUF1259 domain-containing protein	-
FOC64_RS12175	2532093..2532404	-	312	WP_003403164.1	hypothetical protein	-
FOC64_RS12180	2532461..2533222	+	762	WP_003403148.1	two-component system response regulator TcrA	-
FOC64_RS12185	2533266..2533736	+	471	WP_003403144.1	HAMP domain-containing protein	-
FOC64_RS12190	2533850..2534356	+	507	WP_003900973.1	two component system sensor kinase HK1	-
FOC64_RS12195	2534460..2534696	+	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FOC64_RS12200	2534693..2535106	+	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC64_RS12205	2535357..2536592	+	1236	WP_003403128.1	ATP-binding protein	-
FOC64_RS12210	2536775..2537032	+	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
FOC64_RS12215	2537029..2537421	+	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
FOC64_RS12220	2537473..2539023	-	1551	WP_003905349.1	MCE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T158497 WP_003403137.1 NZ_CP054014:2534693-2535106 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T158497 NZ_CP069646:c3884121-3883747 [Escherichia coli O7:H4]
ATGAACACATTACCCGACACACACGTACGGAAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT158497 WP_003403139.1 NZ_CP054014:2534460-2534696 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT158497 NZ_CP069646:c3884579-3884211 [Escherichia coli O7:H4]
GTGTCAGACACATTCTCCGGGACAACTCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCAGCACCGTGAC
GCCCTGTTTCGGGGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAAGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGTTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGCTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTTGCTGTTTATCCGACGCCCGAAACGAAACAGTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89