Detailed information of TA system TA158487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2216709..2217328
• Replicon: chromosome
• Accession: NZ_CP054014
• Organism: Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FOC64_RS10535
• Protein ID: WP_003404726.1
• Coordinates: 2216709..2217143 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: FOC64_RS10540
• Protein ID: WP_003898641.1
• Coordinates: 2217149..2217328 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC64_RS10510	2212044..2213282	+	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-
FOC64_RS10515	2213284..2214792	+	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FOC64_RS10520	2214959..2215189	+	231	WP_003898642.1	hypothetical protein	-
FOC64_RS10525	2215324..2215773	-	450	WP_003404738.1	hypothetical protein	-
FOC64_RS10530	2215838..2216611	-	774	WP_003404735.1	VOC family protein	-
FOC64_RS10535	2216709..2217143	-	435	WP_003404726.1	SRPBCC family protein	Toxin
FOC64_RS10540	2217149..2217328	-	180	WP_003898641.1	antitoxin	Antitoxin
FOC64_RS10545	2217454..2217612	-	159	WP_003404720.1	hypothetical protein	-
FOC64_RS10550	2217885..2220278	-	2394	WP_003898639.1	cation-translocating P-type ATPase	-
FOC64_RS10555	2220275..2221855	-	1581	WP_003910977.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T158487 WP_003404726.1 NZ_CP054014:c2217143-2216709 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T158487 NZ_CP069646:1751702-1752244 [Escherichia coli O7:H4]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT158487 WP_003898641.1 NZ_CP054014:c2217328-2217149 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT158487 NZ_CP069646:1751407-1751718 [Escherichia coli O7:H4]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5