Detailed information of TA system TA158482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1844145..1844704
• Replicon: chromosome
• Accession: NZ_CP054014
• Organism: Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: FOC64_RS08790
• Protein ID: WP_003898789.1
• Coordinates: 1844411..1844704 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: FOC64_RS08785
• Protein ID: WP_003406322.1
• Coordinates: 1844145..1844414 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC64_RS08775	1839407..1840195	+	789	WP_003406325.1	hypothetical protein	-
FOC64_RS08780	1840337..1844032	+	3696	WP_003406323.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
FOC64_RS08785	1844145..1844414	+	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FOC64_RS08790	1844411..1844704	+	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FOC64_RS08795	1844761..1845591	+	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
FOC64_RS08800	1845672..1846532	-	861	WP_003900301.1	hypothetical protein	-
FOC64_RS08805	1846712..1848295	+	1584	WP_009940130.1	PE family protein	-
FOC64_RS08810	1848318..1848749	-	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
FOC64_RS08815	1848746..1849006	-	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T158482 WP_003898789.1 NZ_CP054014:1844411-1844704 [Mycobacterium tuberculosis]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T158482 NZ_CP069646:c719414-719238 [Escherichia coli O7:H4]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT158482 WP_003406322.1 NZ_CP054014:1844145-1844414 [Mycobacterium tuberculosis]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT158482 NZ_CP069646:c719192-718776 [Escherichia coli O7:H4]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2