Detailed information of TA system TA158476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1047665..1048368
• Replicon: chromosome
• Accession: NZ_CP054014
• Organism: Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FOC64_RS05250
• Protein ID: WP_003409778.1
• Coordinates: 1048039..1048368 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FOC64_RS05245
• Protein ID: WP_003409780.1
• Coordinates: 1047665..1048042 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC64_RS05205	1042823..1043014	+	192	WP_003409876.1	hypothetical protein	-
FOC64_RS05210	1043035..1043937	+	903	WP_003899097.1	hypothetical protein	-
FOC64_RS05215	1043948..1044298	+	351	WP_003409871.1	hypothetical protein	-
FOC64_RS05220	1044412..1044594	+	183	WP_003409870.1	hypothetical protein	-
FOC64_RS05225	1044587..1044988	-	402	WP_003409869.1	hypothetical protein	-
FOC64_RS05230	1045052..1045504	+	453	WP_003899095.1	lipoprotein	-
FOC64_RS05235	1045659..1047023	-	1365	WP_003903691.1	HNH endonuclease	-
FOC64_RS05240	1047078..1047668	+	591	WP_003409784.1	SEC-C domain-containing protein	-
FOC64_RS05245	1047665..1048042	+	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FOC64_RS05250	1048039..1048368	+	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOC64_RS05255	1048578..1049348	-	771	WP_003409775.1	SDR family oxidoreductase	-
FOC64_RS05260	1049345..1050406	-	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FOC64_RS05265	1050403..1050918	-	516	WP_003409718.1	flavin reductase family protein	-
FOC64_RS05270	1050915..1051985	-	1071	WP_003902252.1	epoxide hydrolase EphB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T158476 WP_003409778.1 NZ_CP054014:1048039-1048368 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T158476 NZ_CP069642:c4039655-4039437 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT158476 WP_003409780.1 NZ_CP054014:1047665-1048042 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT158476 NZ_CP069642:c4040055-4039681 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8