Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1003625..1004211 Replicon chromosome
Accession NZ_CP054014
Organism Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag FOC64_RS04945 Protein ID WP_003410010.1
Coordinates 1003867..1004211 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag FOC64_RS04940 Protein ID WP_003410014.1
Coordinates 1003625..1003873 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC64_RS04920 999608..1000375 - 768 WP_003410019.1 hemerythrin domain-containing protein -
FOC64_RS04925 1000532..1000888 + 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
FOC64_RS04930 1000941..1001213 + 273 WP_003410017.1 DUF1490 family protein -
FOC64_RS04935 1001210..1003525 + 2316 WP_003899120.1 cation transporter ATPase CptG -
FOC64_RS04940 1003625..1003873 + 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
FOC64_RS04945 1003867..1004211 + 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
FOC64_RS04950 1004300..1004635 + 336 WP_003410009.1 dehydrogenase -
FOC64_RS04955 1004536..1004793 - 258 WP_003410006.1 hypothetical protein -
FOC64_RS04960 1004879..1005220 + 342 WP_003410003.1 DUF2384 domain-containing protein -
FOC64_RS04965 1005217..1005777 + 561 WP_003410001.1 RES family NAD+ phosphorylase -
FOC64_RS04970 1006297..1006836 - 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
FOC64_RS04975 1007062..1007490 - 429 WP_003409992.1 cellulose-binding protein -
FOC64_RS04980 1007906..1008505 - 600 WP_003910883.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T158471 WP_003410010.1 NZ_CP054014:1003867-1004211 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T158471 NZ_CP069642:3360417-3360794 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT158471 WP_003410014.1 NZ_CP054014:1003625-1003873 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT158471 NZ_CP069642:3359959-3360327 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References