Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 910693..911329 Replicon chromosome
Accession NZ_CP054014
Organism Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag FOC64_RS04540 Protein ID WP_003410654.1
Coordinates 910693..911103 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag FOC64_RS04545 Protein ID WP_003410651.1
Coordinates 911096..911329 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC64_RS04520 906290..907513 + 1224 WP_003901321.1 class I SAM-dependent methyltransferase -
FOC64_RS04525 907459..908985 - 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
FOC64_RS04530 908982..909608 - 627 WP_003410658.1 precorrin-8X methylmutase -
FOC64_RS04535 909618..910709 - 1092 WP_003900454.1 precorrin-3B synthase -
FOC64_RS04540 910693..911103 - 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
FOC64_RS04545 911096..911329 - 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
FOC64_RS04550 911407..914991 + 3585 WP_003910889.1 cobaltochelatase subunit CobN -
FOC64_RS04555 915075..915479 + 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
FOC64_RS04560 915480..915881 - 402 WP_009937711.1 metal ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T158468 WP_003410654.1 NZ_CP054014:c911103-910693 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T158468 NZ_CP069642:c2447851-2447744 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT158468 WP_003410651.1 NZ_CP054014:c911329-911096 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT158468 NZ_CP069642:2447899-2447965 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References