Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 364708..365339 Replicon chromosome
Accession NZ_CP054014
Organism Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag FOC64_RS01945 Protein ID WP_003413174.1
Coordinates 364708..365085 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag FOC64_RS01950 Protein ID WP_003413167.1
Coordinates 365082..365339 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC64_RS01910 360175..360687 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
FOC64_RS01915 360680..361933 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
FOC64_RS01920 361930..362739 + 810 WP_003413193.1 shikimate dehydrogenase -
FOC64_RS01925 362751..363170 + 420 WP_003413190.1 A24 family peptidase -
FOC64_RS01930 363581..363826 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
FOC64_RS01935 363823..364218 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
FOC64_RS01940 364318..364692 + 375 WP_003413177.1 hypothetical protein -
FOC64_RS01945 364708..365085 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
FOC64_RS01950 365082..365339 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
FOC64_RS01955 365378..365791 - 414 WP_003413164.1 PIN domain nuclease -
FOC64_RS01960 365884..366161 - 278 Protein_387 type II toxin-antitoxin system VapB family antitoxin -
FOC64_RS01965 366158..366820 - 663 WP_003905878.1 hypothetical protein -
FOC64_RS01970 366817..367476 - 660 WP_003902296.1 hypothetical protein -
FOC64_RS01975 367603..368814 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -
FOC64_RS01980 369110..369424 - 315 WP_009937839.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T158462 WP_003413174.1 NZ_CP054014:c365085-364708 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T158462 NZ_CP069642:c739442-739188 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT158462 WP_003413167.1 NZ_CP054014:c365339-365082 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT158462 NZ_CP069642:c739690-739439 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References