Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 363581..364218 Replicon chromosome
Accession NZ_CP054014
Organism Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FOC64_RS01935 Protein ID WP_003413180.1
Coordinates 363823..364218 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FOC64_RS01930 Protein ID WP_003413183.1
Coordinates 363581..363826 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC64_RS01910 360175..360687 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
FOC64_RS01915 360680..361933 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
FOC64_RS01920 361930..362739 + 810 WP_003413193.1 shikimate dehydrogenase -
FOC64_RS01925 362751..363170 + 420 WP_003413190.1 A24 family peptidase -
FOC64_RS01930 363581..363826 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FOC64_RS01935 363823..364218 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FOC64_RS01940 364318..364692 + 375 WP_003413177.1 hypothetical protein -
FOC64_RS01945 364708..365085 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FOC64_RS01950 365082..365339 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FOC64_RS01955 365378..365791 - 414 WP_003413164.1 PIN domain nuclease -
FOC64_RS01960 365884..366161 - 278 Protein_387 type II toxin-antitoxin system VapB family antitoxin -
FOC64_RS01965 366158..366820 - 663 WP_003905878.1 hypothetical protein -
FOC64_RS01970 366817..367476 - 660 WP_003902296.1 hypothetical protein -
FOC64_RS01975 367603..368814 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T158461 WP_003413180.1 NZ_CP054014:363823-364218 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T158461 NZ_CP069642:727100-727474 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT158461 WP_003413183.1 NZ_CP054014:363581-363826 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT158461 NZ_CP069642:726643-727011 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References