Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 164207..164886 Replicon chromosome
Accession NZ_CP054014
Organism Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag FOC64_RS00855 Protein ID WP_003414059.1
Coordinates 164470..164886 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FOC64_RS00850 Protein ID WP_003414061.1
Coordinates 164207..164473 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC64_RS00815 159683..160420 - 738 WP_003414079.1 dienelactone hydrolase family protein -
FOC64_RS00820 160576..161376 + 801 WP_003911953.1 thymidylate synthase -
FOC64_RS00825 161447..161926 + 480 WP_003414073.1 dihydrofolate reductase -
FOC64_RS00830 162000..162419 + 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FOC64_RS00835 162416..163510 + 1095 WP_003414068.1 restriction endonuclease subunit S -
FOC64_RS00840 163520..163789 + 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FOC64_RS00845 163786..164181 + 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
FOC64_RS00850 164207..164473 + 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FOC64_RS00855 164470..164886 + 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
FOC64_RS00860 164973..166595 + 1623 WP_003414057.1 type I restriction-modification system subunit M -
FOC64_RS00865 166592..166867 + 276 WP_003414055.1 type I restriction endonuclease subunit S -
FOC64_RS00870 167111..167863 + 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FOC64_RS00875 167932..168834 + 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T158458 WP_003414059.1 NZ_CP054014:164470-164886 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T158458 NZ_CP069642:c68761-68483 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT158458 WP_003414061.1 NZ_CP054014:164207-164473 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT158458 NZ_CP069642:c68483-68199 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References