Detailed information of TA system TA158457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 163786..164473
• Replicon: chromosome
• Accession: NZ_CP054014
• Organism: Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: FOC64_RS00845
• Protein ID: WP_003414064.1
• Coordinates: 163786..164181 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FOC64_RS00850
• Protein ID: WP_003414061.1
• Coordinates: 164207..164473 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC64_RS00815	159683..160420	-	738	WP_003414079.1	dienelactone hydrolase family protein	-
FOC64_RS00820	160576..161376	+	801	WP_003911953.1	thymidylate synthase	-
FOC64_RS00825	161447..161926	+	480	WP_003414073.1	dihydrofolate reductase	-
FOC64_RS00830	162000..162419	+	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FOC64_RS00835	162416..163510	+	1095	WP_003414068.1	restriction endonuclease subunit S	-
FOC64_RS00840	163520..163789	+	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FOC64_RS00845	163786..164181	+	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC64_RS00850	164207..164473	+	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FOC64_RS00855	164470..164886	+	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
FOC64_RS00860	164973..166595	+	1623	WP_003414057.1	type I restriction-modification system subunit M	-
FOC64_RS00865	166592..166867	+	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FOC64_RS00870	167111..167863	+	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FOC64_RS00875	167932..168834	+	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T158457 WP_003414064.1 NZ_CP054014:163786-164181 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T158457 NZ_CP069640:1791-2075 [Escherichia coli]
ATGAGCTATAACCTTGAATTTGATCCCCGAGCCCTGAAGGAATGGCGCAAGCTCGGGGATGATGTCCGTCTGCAGTTCAA
GAAAAAACTCGAGCAGGTTCTACAACACCCGCGGATCGATAAAAATCGCCTGCGAGAGCTGCATGACTGCTACAAAATCA
AGCTCCGTGCATCCGGTTATCGCTTGGTCTATCAGGTTCGCGATCAAACCATTACGGTATTCGTGGTGGCGGTCGGTAAG
CGCGAGCGTTCTGCCGCTTACGATGCGGCCGATAAACGCTTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT158457 WP_003414061.1 NZ_CP054014:164207-164473 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT158457 NZ_CP069640:1559-1801 [Escherichia coli]
ATGGCCACGATTAATATTCGGATCGATGACGAGCTGAAAAGCCGCTCTTATGCCGCACTGGAAAAGCTGGGCGTAACGCC
GTCCGAGGTTCTGCGCCAAACACTGGAATATGTGGCCCAAAGCGGACGTTTGCCGTTCCAGCAGGTTTTGCTGACCGAGG
ATGATGCCGATTTGATGGCTATCGTTCGGGATCGTCTGGAAAACCCACAGGCGGGCGTAAAGGTGTCACTGGATGAGCTA
TAA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64