Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 124319..124889 Replicon chromosome
Accession NZ_CP054014
Organism Mycobacterium tuberculosis strain FDAARGOS_756

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FOC64_RS00635 Protein ID WP_003414166.1
Coordinates 124533..124889 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FOC64_RS00630 Protein ID WP_003901465.1
Coordinates 124319..124549 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC64_RS00590 119337..119648 - 312 WP_003414190.1 hypothetical protein -
FOC64_RS00595 119753..120010 - 258 WP_003899489.1 hypothetical protein -
FOC64_RS00600 120244..121398 - 1155 WP_003899488.1 DDE-type integrase/transposase/recombinase -
FOC64_RS00605 121597..121788 - 192 WP_003414184.1 hypothetical protein -
FOC64_RS00610 121785..122189 - 405 WP_003414181.1 hypothetical protein -
FOC64_RS00615 122337..122591 + 255 WP_003917684.1 hypothetical protein -
FOC64_RS00620 122767..123042 - 276 WP_003911993.1 DUF1778 domain-containing protein -
FOC64_RS00625 123233..124276 + 1044 WP_003414172.1 DUF2293 domain-containing protein -
FOC64_RS00630 124319..124549 + 231 WP_003901465.1 antitoxin MazE Antitoxin
FOC64_RS00635 124533..124889 + 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FOC64_RS00640 124991..126640 - 1650 WP_003899485.1 CocE/NonD family hydrolase -
FOC64_RS00645 126659..127288 - 630 WP_003414158.1 DUF3558 family protein -
FOC64_RS00650 127419..127745 + 327 WP_003414157.1 hypothetical protein -
FOC64_RS00655 127749..129437 + 1689 WP_003414155.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T158456 WP_003414166.1 NZ_CP054014:124533-124889 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T158456 NZ_CP069639:c4881537-4881430 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTTGGCTGGTGGCGTAGCCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT158456 WP_003901465.1 NZ_CP054014:124319-124549 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT158456 NZ_CP069639:4881593-4881654 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTGACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References