Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3954334..3954904 Replicon chromosome
Accession NZ_CP054013
Organism Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag FOC65_RS18515 Protein ID WP_003414166.1
Coordinates 3954334..3954690 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag FOC65_RS18520 Protein ID WP_003901465.1
Coordinates 3954674..3954904 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC65_RS18495 3949786..3951474 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
FOC65_RS18500 3951478..3951804 - 327 WP_003414157.1 hypothetical protein -
FOC65_RS18505 3951935..3952564 + 630 WP_003414158.1 DUF3558 family protein -
FOC65_RS18510 3952583..3954232 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
FOC65_RS18515 3954334..3954690 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
FOC65_RS18520 3954674..3954904 - 231 WP_003901465.1 antitoxin MazE Antitoxin
FOC65_RS18525 3954947..3955990 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
FOC65_RS18530 3956181..3956456 + 276 WP_003911993.1 DUF1778 domain-containing protein -
FOC65_RS18535 3956632..3956886 - 255 WP_003917684.1 hypothetical protein -
FOC65_RS18540 3957034..3957438 + 405 WP_009938577.1 hypothetical protein -
FOC65_RS18545 3957435..3957626 + 192 WP_003414184.1 hypothetical protein -
FOC65_RS18550 3957825..3958979 + 1155 WP_003911958.1 DDE-type integrase/transposase/recombinase -
FOC65_RS18555 3959213..3959470 + 258 WP_003899489.1 hypothetical protein -
FOC65_RS18560 3959575..3959886 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T158446 WP_003414166.1 NZ_CP054013:c3954690-3954334 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T158446 NZ_CP069639:c2447851-2447744 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT158446 WP_003901465.1 NZ_CP054013:c3954904-3954674 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT158446 NZ_CP069639:2447899-2447965 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References