Detailed information of TA system TA158444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3914337..3915016
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: FOC65_RS18295
• Protein ID: WP_003414059.1
• Coordinates: 3914337..3914753 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: FOC65_RS18300
• Protein ID: WP_003414061.1
• Coordinates: 3914750..3915016 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS18275	3910389..3911291	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FOC65_RS18280	3911360..3912112	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
FOC65_RS18285	3912356..3912631	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
FOC65_RS18290	3912628..3914250	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
FOC65_RS18295	3914337..3914753	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
FOC65_RS18300	3914750..3915016	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FOC65_RS18305	3915042..3915437	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
FOC65_RS18310	3915434..3915703	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
FOC65_RS18315	3915713..3916807	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
FOC65_RS18320	3916804..3917223	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
FOC65_RS18325	3917297..3917776	-	480	WP_003414073.1	dihydrofolate reductase	-
FOC65_RS18330	3917847..3918647	-	801	WP_003911953.1	thymidylate synthase	-
FOC65_RS18335	3918803..3919540	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T158444 WP_003414059.1 NZ_CP054013:c3914753-3914337 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T158444 NZ_CP069639:1802210-1802587 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT158444 WP_003414061.1 NZ_CP054013:c3915016-3914750 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT158444 NZ_CP069639:1801753-1802121 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64