Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3713894..3714531 Replicon chromosome
Accession NZ_CP054013
Organism Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag FOC65_RS17220 Protein ID WP_003413180.1
Coordinates 3713894..3714289 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag FOC65_RS17225 Protein ID WP_003413183.1
Coordinates 3714286..3714531 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC65_RS17180 3709297..3710508 + 1212 WP_009936235.1 alpha/beta hydrolase family protein -
FOC65_RS17185 3710635..3711294 + 660 WP_003900846.1 LppA family lipoprotein -
FOC65_RS17190 3711291..3711953 + 663 WP_003900848.1 hypothetical protein -
FOC65_RS17195 3711950..3712228 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
FOC65_RS17200 3712321..3712734 + 414 WP_003413164.1 PIN domain nuclease -
FOC65_RS17205 3712773..3713030 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
FOC65_RS17210 3713027..3713404 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
FOC65_RS17215 3713420..3713794 - 375 WP_003413177.1 hypothetical protein -
FOC65_RS17220 3713894..3714289 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
FOC65_RS17225 3714286..3714531 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
FOC65_RS17230 3714942..3715361 - 420 WP_003413190.1 A24 family peptidase -
FOC65_RS17235 3715373..3716182 - 810 WP_003413193.1 shikimate dehydrogenase -
FOC65_RS17240 3716179..3717432 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
FOC65_RS17245 3717425..3717937 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T158441 WP_003413180.1 NZ_CP054013:c3714289-3713894 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T158441 NZ_CP069639:c739690-739439 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT158441 WP_003413183.1 NZ_CP054013:c3714531-3714286 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT158441 NZ_CP069639:c739442-739188 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References