Detailed information of TA system TA158440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3712773..3713404
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FOC65_RS17210
• Protein ID: WP_003413174.1
• Coordinates: 3713027..3713404 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FOC65_RS17205
• Protein ID: WP_003413167.1
• Coordinates: 3712773..3713030 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS17175	3708594..3709001	+	408	WP_003413029.1	hypothetical protein	-
FOC65_RS17180	3709297..3710508	+	1212	WP_009936235.1	alpha/beta hydrolase family protein	-
FOC65_RS17185	3710635..3711294	+	660	WP_003900846.1	LppA family lipoprotein	-
FOC65_RS17190	3711291..3711953	+	663	WP_003900848.1	hypothetical protein	-
FOC65_RS17195	3711950..3712228	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FOC65_RS17200	3712321..3712734	+	414	WP_003413164.1	PIN domain nuclease	-
FOC65_RS17205	3712773..3713030	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FOC65_RS17210	3713027..3713404	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC65_RS17215	3713420..3713794	-	375	WP_003413177.1	hypothetical protein	-
FOC65_RS17220	3713894..3714289	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FOC65_RS17225	3714286..3714531	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FOC65_RS17230	3714942..3715361	-	420	WP_003413190.1	A24 family peptidase	-
FOC65_RS17235	3715373..3716182	-	810	WP_003413193.1	shikimate dehydrogenase	-
FOC65_RS17240	3716179..3717432	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
FOC65_RS17245	3717425..3717937	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T158440 WP_003413174.1 NZ_CP054013:3713027-3713404 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T158440 NZ_CP069639:c739442-739188 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT158440 WP_003413167.1 NZ_CP054013:3712773-3713030 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT158440 NZ_CP069639:c739690-739439 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5