Detailed information of TA system TA158430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 3074840..3075738
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: FOC65_RS14200
• Protein ID: WP_003410001.1
• Coordinates: 3074840..3075400 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: FOC65_RS14205
• Protein ID: WP_003410003.1
• Coordinates: 3075397..3075738 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS14170	3070009..3070662	-	654	WP_003409976.1	cutinase Cfp21	-
FOC65_RS14175	3070777..3071007	+	231	WP_003409980.1	DUF167 domain-containing protein	-
FOC65_RS14180	3071092..3072003	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
FOC65_RS14185	3072112..3072711	+	600	WP_003409989.1	L-lysine exporter	-
FOC65_RS14190	3073127..3073555	+	429	WP_003409992.1	cellulose-binding protein	-
FOC65_RS14195	3073781..3074320	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
FOC65_RS14200	3074840..3075400	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
FOC65_RS14205	3075397..3075738	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
FOC65_RS14210	3075824..3076081	+	258	WP_003410006.1	hypothetical protein	-
FOC65_RS14215	3075982..3076317	-	336	WP_003410009.1	dehydrogenase	-
FOC65_RS14220	3076406..3076750	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
FOC65_RS14225	3076744..3076992	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
FOC65_RS14230	3077092..3079407	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
FOC65_RS14235	3079404..3079676	-	273	WP_003410017.1	DUF1490 family protein	-
FOC65_RS14240	3079729..3080085	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T158430 WP_003410001.1 NZ_CP054013:c3075400-3074840 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T158430 NZ_CP069636:3547829-3548143 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT158430 WP_003410003.1 NZ_CP054013:c3075738-3075397 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT158430 NZ_CP069636:3547593-3547826 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• PDB: 6FKG

Antitoxin

• AlphaFold DB: A0A7U4FAE4