Detailed information of TA system TA158426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3036744..3037447
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: FOC65_RS13945
• Protein ID: WP_003409778.1
• Coordinates: 3036744..3037073 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: FOC65_RS13950
• Protein ID: WP_003409780.1
• Coordinates: 3037070..3037447 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS13925	3033127..3034197	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
FOC65_RS13930	3034194..3034709	+	516	WP_003409718.1	flavin reductase family protein	-
FOC65_RS13935	3034706..3035767	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
FOC65_RS13940	3035764..3036534	+	771	WP_003409775.1	SDR family oxidoreductase	-
FOC65_RS13945	3036744..3037073	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOC65_RS13950	3037070..3037447	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
FOC65_RS13955	3037444..3038034	-	591	WP_003409784.1	SEC-C domain-containing protein	-
FOC65_RS13960	3038089..3039453	+	1365	WP_003903691.1	HNH endonuclease	-
FOC65_RS13965	3039608..3040060	-	453	WP_003899095.1	lipoprotein	-
FOC65_RS13970	3040124..3040525	+	402	WP_003409869.1	hypothetical protein	-
FOC65_RS13975	3040518..3040700	-	183	WP_003409870.1	hypothetical protein	-
FOC65_RS13980	3040814..3041164	-	351	WP_003899096.1	hypothetical protein	-
FOC65_RS13985	3041175..3042077	-	903	WP_003899097.1	hypothetical protein	-
FOC65_RS13990	3042098..3042289	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T158426 WP_003409778.1 NZ_CP054013:c3037073-3036744 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T158426 NZ_CP069636:3172060-3172233 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT158426 WP_003409780.1 NZ_CP054013:c3037447-3037070 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT158426 NZ_CP069636:3171736-3172032 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8