Detailed information of TA system TA158419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2224813..2225501
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: FOC65_RS10310
• Protein ID: WP_003406304.1
• Coordinates: 2225070..2225501 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: FOC65_RS10305
• Protein ID: WP_003406302.1
• Coordinates: 2224813..2225073 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS10285	2220390..2221214	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
FOC65_RS10290	2221219..2222400	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
FOC65_RS10295	2222477..2223577	-	1101	WP_003916789.1	magnesium/cobalt transporter CorA	-
FOC65_RS10300	2223748..2224737	+	990	WP_003406301.1	malate dehydrogenase	-
FOC65_RS10305	2224813..2225073	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
FOC65_RS10310	2225070..2225501	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC65_RS10315	2225524..2227212	-	1689	WP_003910308.1	PE family protein	-
FOC65_RS10320	2227392..2228252	+	861	WP_003900301.1	hypothetical protein	-
FOC65_RS10325	2228333..2229163	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
FOC65_RS10330	2229220..2229513	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
FOC65_RS10335	2229510..2229779	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T158419 WP_003406304.1 NZ_CP054013:2225070-2225501 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T158419 NZ_CP069636:c739690-739439 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT158419 WP_003406302.1 NZ_CP054013:2224813-2225073 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT158419 NZ_CP069636:c739442-739188 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6