Detailed information of TA system TA158418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2079950..2080518
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FOC65_RS09665
• Protein ID: WP_003405865.1
• Coordinates: 2080144..2080518 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FOC65_RS09660
• Protein ID: WP_003405863.1
• Coordinates: 2079950..2080147 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS09640	2075991..2076629	-	639	WP_003898730.1	lipid droplet-associated protein	-
FOC65_RS09645	2076719..2077726	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FOC65_RS09650	2077743..2078726	-	984	WP_003898731.1	hypothetical protein	-
FOC65_RS09655	2078789..2079862	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FOC65_RS09660	2079950..2080147	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FOC65_RS09665	2080144..2080518	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC65_RS09670	2080721..2081419	+	699	WP_003898733.1	hypothetical protein	-
FOC65_RS09675	2081537..2081722	+	186	WP_003901093.1	hypothetical protein	-
FOC65_RS09680	2081649..2081924	-	276	WP_003405867.1	hypothetical protein	-
FOC65_RS09685	2082167..2082490	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FOC65_RS09690	2082505..2083215	-	711	WP_003911399.1	hypothetical protein	-
FOC65_RS09695	2083237..2083446	+	210	WP_003911400.1	hypothetical protein	-
FOC65_RS09700	2083544..2084038	-	495	WP_003405876.1	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T158418 WP_003405865.1 NZ_CP054013:2080144-2080518 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T158418 NZ_CP069636:c739442-739188 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT158418 WP_003405863.1 NZ_CP054013:2079950-2080147 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT158418 NZ_CP069636:c739690-739439 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07