Detailed information of TA system TA158414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1855214..1855833
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FOC65_RS08580
• Protein ID: WP_003404726.1
• Coordinates: 1855399..1855833 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: FOC65_RS08575
• Protein ID: WP_003898641.1
• Coordinates: 1855214..1855393 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS08560	1850687..1852267	+	1581	WP_003909313.1	serine hydrolase	-
FOC65_RS08565	1852264..1854657	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
FOC65_RS08570	1854930..1855088	+	159	WP_003404720.1	hypothetical protein	-
FOC65_RS08575	1855214..1855393	+	180	WP_003898641.1	antitoxin	Antitoxin
FOC65_RS08580	1855399..1855833	+	435	WP_003404726.1	SRPBCC family protein	Toxin
FOC65_RS08585	1855931..1856704	+	774	WP_003404735.1	VOC family protein	-
FOC65_RS08590	1856769..1857218	+	450	WP_003404738.1	hypothetical protein	-
FOC65_RS08595	1857353..1857583	-	231	WP_003898642.1	hypothetical protein	-
FOC65_RS08600	1857750..1859258	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FOC65_RS08605	1859260..1860498	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T158414 WP_003404726.1 NZ_CP054013:1855399-1855833 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T158414 NZ_CP069636:c68761-68483 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT158414 WP_003898641.1 NZ_CP054013:1855214-1855393 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT158414 NZ_CP069636:c68483-68199 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5