Detailed information of TA system TA158409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1593516..1594149
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: FOC65_RS07245
• Protein ID: WP_003403365.1
• Coordinates: 1593516..1593899 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: FOC65_RS07250
• Protein ID: WP_003403368.1
• Coordinates: 1593994..1594149 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS07220	1588808..1589344	+	537	WP_003403341.1	50S ribosomal protein L10	-
FOC65_RS07225	1589381..1589773	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
FOC65_RS07230	1589766..1590461	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
FOC65_RS07235	1590532..1592037	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FOC65_RS07240	1592118..1593128	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FOC65_RS07245	1593516..1593899	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
FOC65_RS07250	1593994..1594149	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FOC65_RS07255	1594225..1594941	-	717	WP_010886082.1	CPBP family intramembrane metalloprotease	-
FOC65_RS07260	1595217..1595525	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FOC65_RS07265	1595512..1595757	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
FOC65_RS07270	1595867..1596304	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FOC65_RS07275	1596301..1596555	-	255	WP_003911263.1	antitoxin VapB7	-
FOC65_RS07280	1596669..1599032	+	2364	WP_003898547.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T158409 WP_003403365.1 NZ_CP054013:c1593899-1593516 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T158409 NZ_CP069633:c3746968-3746591 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT158409 WP_003403368.1 NZ_CP054013:c1594149-1593994 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT158409 NZ_CP069633:c3747389-3747015 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8