Detailed information of TA system TA158405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1543776..1544419
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: FOC65_RS06970
• Protein ID: WP_003403187.1
• Coordinates: 1544018..1544419 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: FOC65_RS06965
• Protein ID: WP_003403184.1
• Coordinates: 1543776..1544021 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS06930	1539056..1539526	-	471	WP_003403144.1	HAMP domain-containing protein	-
FOC65_RS06935	1539570..1540331	-	762	WP_003403148.1	two-component system response regulator TcrA	-
FOC65_RS06940	1540388..1540699	+	312	WP_003403164.1	hypothetical protein	-
FOC65_RS06945	1540771..1541721	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
FOC65_RS06950	1541962..1542546	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FOC65_RS06955	1542548..1543258	+	711	Protein_1377	transposase	-
FOC65_RS06960	1543345..1543731	+	387	WP_003403180.1	hypothetical protein	-
FOC65_RS06965	1543776..1544021	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FOC65_RS06970	1544018..1544419	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC65_RS06975	1544362..1544589	+	228	WP_003900974.1	hypothetical protein	-
FOC65_RS06980	1544807..1544974	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
FOC65_RS06985	1545007..1545204	-	198	WP_003403191.1	hypothetical protein	-
FOC65_RS06990	1545284..1546441	-	1158	WP_003898524.1	hypothetical protein	-
FOC65_RS06995	1546493..1546714	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
FOC65_RS07000	1546856..1547461	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T158405 WP_003403187.1 NZ_CP054013:1544018-1544419 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T158405 NZ_CP069633:3360416-3360793 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT158405 WP_003403184.1 NZ_CP054013:1543776-1544021 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT158405 NZ_CP069633:3359958-3360326 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91